The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. of 0. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Lucid Scientific. SoftMax Pro Software’s. Avoid losing high signal. HPV Serology Laboratory Standard Operating Procedure . The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 2. No additional manuals are available. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. 00 Add to cart; Molecular Devices EMax Microplate Reader $ 1,295. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. 96, 384, 24, 12, 6 well types. . The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 0 OD at. #N207) LAL Reagent Water (Lonza cat. 10. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. section, select plate section titled. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. microplates or in a cuvette at a. 5 mm and 15. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. The VersaMax and SpectraMax 190 read 96-well microplates. Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. • 0. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Infinite 200 Pro Microplate Reader. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 3% in white 96- and. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . CloneMatrix. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Other features and benefits include: Eliminate changing out filters. Or scan up to 384 wells at any time to determine the best assay conditions. . SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. Affordable multi-mode microplate reader with . Photometric and kinetic analysis mode. 03 pM 384-well: 0. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. #SpectraMax L Config) Methods. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Molecular Devices' microplate readers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Reading a Microplate . This reader also makes use of a unique temperature-independent methodology for path length correction. 40266. The system provides options for 20-, 40-,. for ValitaTiter Plus standards read on Molecular Devices readers. A. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. It features a modular design that allows users to add or change detection cartridges according to their needs. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. 1 mL/well of media. Plates can put in the drawer both in landscape and portrait positions. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. 8 Pages. #ID3-STD) L-Tryptophan (Sigma cat. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Use standard plate unless you see your type listed. Molecular Devices' microplate readers. 384-well: 0. Temperature control. 340‚ 850 nm. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. Plate wash time (300 µl). Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Microplates . validation, maintenance, and repair documentation. Figure 2-23: Bottle holder To install the bottle holder: 1. In addition, the iD5 reader measures TRF and FP and. 1536-well: 0. 50 µL of. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. Learn more 0200-7008 6- to 1536-well plates. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Optimized for fluorescence microplate readers. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Related Products: Low Binding Plates. we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert. Specifications for the SpectraMax L microplate reader. 19 H mm that meet microplate industry standards. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. Read time* 96 wells < 15 sec. The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. #0310-5227) 96-well cell wash head (Molecular Devices Cat. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. For more sample throughput, the system can well. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. It also integrates with the powerful SoftMax Pro Software for. 020 fmol 0. It also integrates with the. BSA concentration (µg/mL) Average OD. 2600A October 2023. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. fluorescence microplate reader from Molecular Devices. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. # 0310-5218T). Endpoint, kinetic, spectrum and area-well scanning Click here. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). OEM Molecular Devices All Microplate Reader, Top r. #L6876) Guanidine hydrochloride (Sigma cat. The contents of the SpectraDrop Microplate kit depend on kit type. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Read the microplate Step 1: Place the microplate in the reader. Triplicates of each BSA concentration were made for this assay plate. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Efficient cell washing with minimal cell loss and optimal assay performance. Wavelength Range. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 6 L (with SpectraMax i3x System) Title: SpectraMax MiniMax 300 Imaging Cytometer | Molecular. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Category 2 and 3 microplate readers use gradient heating as well. This is equivalent to having 511 built-in filters,Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. Cross-talk < 0. The Multiskan Sky microplate reader is an absorbance plate reader. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. absorbance, fluorescence, and luminescence with the added functionality of. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. #G7294). Features. Microplate luminescent reader assay for ERK activity. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. SmartReader 96: Designed for optimal ELISA Testing. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. 1. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Softmax Pro GxP Software. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 2 2601A ValitaTiter. i3x. 0 H x 60. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). ± 0. 0 OD Photometric resolution: 0. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. Visible absorbance -. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. #iD5-STD) Methods ATP standard curve. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Consult Molecular Devices's entire Threshold Immunoassay System catalogue on MedicalExpo. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. Measure more accurately. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. If the best wavelengths are unknown, the Gemini EM reader will find. emax and Vmax microplate readers. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Total volume (except deep. explore more. Microplate Reader, Molecular Devices Optimax, Refurbished. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. M5. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. SpectraMax Microplate Readers. ” Promega Corp. SOP Title: Use and. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Ordering information FlexStation 3 Microplate Reader Part Number: FLEX3 • FlexStation 3 Base System Consumables 96-Well, FlexStation Pipet Tips (Black) Part Number: 9000-0911 • 200 µL capacity • Includes 3 vials each of FLIPR Calcium 6 and 6-QF dyes Reagents FLIPR® Calcium 6 Assay Evaluation Kit Part Number. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Description. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. SpectraMax® 340PC384 Microplate Reader for. Place the microplate in the drawer. SoftMax Pro Software’s SpectralThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 39. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Jon Cuccui, Brendan W. 03 pM 384-well: 0. Key applications: ELISA, nucleic acid, endotoxin testing. Microplate Readers. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Fluorescence Microplate Reader Comparison Chart. Page 154: Ordering Parts And Accessories. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The iMark. #W50-100) SpectraMax® ABS Plus Microplate Reader. Published Dec 16, 2020 | Updated Dec 16, 2020. 1 FP • Fluorescence Polarization detection for fluorescein- or . We would like to show you a description here but the site won’t allow us. 04 sec. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. Reconstitution of reagents. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. For more sample throughput, both readers can be easily integrated into full roboticThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Industry standard, 96-well plates are. 2 mm x 86 mm) Height: between 13. 100 μL/well Steady-Luc working solution was added to the wells. M3-M5e_manual. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. The plate reader optics are tunable for. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Contents of wells were pipetted up and down several times to mix. Read one sample or up to 384 in a. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. The SpectraMax ABS. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. Insert the filled microplate into the drawer, matching well A1 with position A1. 1/17 1907B Printed in USA . the appropriate well of the microplate. 2022 Harini Natarajan et al. Place the microplate in the drawer. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Keywords: fluorescence polarization, trf, fret, igg. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. reagent basin for ease of pipetting into. The color touch screen provides a visual user interface, making programming fast and intuitive. Seller assumes all responsibility for this listing. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. Integrated stacker option. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. These instruments are all elegant and simple in their form and function. Click on the crosshair and move it so that it is centered over the well image. 500 USD. 3:40 . Reactions catalyzed by firefly and *Renilla luciferases. Three kits covering a wide range of dsDNA concentrations. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. About;. Incubate the plate at 37 °C for approximately one hour. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Microplate readers use a vertical light path so the distance of the. Plate readers, also known as microplate readers or microplate photometers,. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. 92: 88. 76 L x 85. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. After 5, 7, 11, and 13 days. Optical System: High energy xenon flash lamp. Fluorescence Microplate Reader Comparison Chart. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Model:. 3. Temperature Control: Ambient +5C up to 42C. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. We would like to show you a description here but the site won’t allow us. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. 96-well flat clear bottom white TC-treated microplates (Corning cat. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Quantitate proteins rapidly with a 5-minute, room-temperature incubation. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The VersaMax reads 96-well microplates. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. 2 OD at 200 nm. 300 µL of Detergent Compatible Bradford reagent was added to each well. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. Fluorescence Microplate Reader Comparison Chart. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. LVF Monochromator™ : Excitation and Emission. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. 50 racks/case for 384-well (16 channel pipettor head. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Outer wells are aligned for proper viewing with FLIPR. Vrla used the device to quantify the concentration of a small molecule produced by. com. 54: 1. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Five-second read per plate. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. #I2511) • Assay diluent – may be cell culture medium. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. It also allows for the elimination of standard curves. Comprehensive data analysis software. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. #M5e) Methods Microplate reader compatibility test.